Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements
WahWah TheinHan1, Jun Liu2, Minghui Tang1
1Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Bone Research
|May 20, 2014
Summary
Induced pluripotent stem cells (iPSCs) can generate mesenchymal stem cells (iPSC-MSCs) that enhance bone regeneration. Biofunctionalized calcium phosphate cement (CPC) significantly boosts iPSC-MSC proliferation and osteogenic differentiation for bone repair.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Regenerative Medicine
Background:
- Induced pluripotent stem cells (iPSCs) offer significant potential for regenerative medicine due to their self-renewal and differentiation capabilities.
- Mesenchymal stem cells (MSCs) are crucial for tissue repair, and iPSC-derived MSCs (iPSC-MSCs) present a promising alternative source.
- Calcium phosphate cement (CPC) is a widely used bone graft material, but its bioactivity can be enhanced.
Purpose of the Study:
- To generate iPSC-MSCs and investigate their proliferation and osteogenic differentiation on biofunctionalized CPC.
- To evaluate the efficacy of incorporating various biofunctional agents (RGD peptides, fibronectin, FEPP, Geltrex, platelet concentrate) into CPC for enhanced cell activity.
- To assess the potential of iPSC-MSC-CPC constructs for bone regeneration applications.
Main Methods:
- Human iPSCs were generated from CD34+ cells and differentiated into iPSC-MSCs via embryoid bodies.
- Five types of biofunctionalized CPCs were prepared by incorporating RGD peptides, fibronectin, FEPP, Geltrex, or platelet concentrate.
- iPSC-MSCs were cultured on the biofunctionalized CPCs, and their proliferation, actin expression, osteogenic differentiation markers (alkaline phosphatase, Runx2, collagen-I), and mineralization were analyzed.
Main Results:
- iPSC-MSCs seeded on biofunctionalized CPCs exhibited significantly enhanced proliferation compared to the control group.
- Osteogenic differentiation was upregulated, evidenced by increased expression of alkaline phosphatase, Runx2, and collagen-I.
- Mineralization was notably improved, with iPSC-MSCs on CPC-Platelets showing a threefold increase in mineral synthesis compared to the control CPC.
Conclusions:
- iPSCs are a viable source for generating MSCs with high potential for bone engineering applications.
- Biofunctionalized CPCs significantly improve iPSC-MSC proliferation, osteogenic differentiation, and mineralization compared to traditional CPC.
- iPSC-MSC-CPC constructs demonstrate considerable promise for promoting bone regeneration in craniofacial and orthopedic repairs.
