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Single channel recordings from basolateral membranes of frog urinary bladders
Acta Physiologica Et Pharmacologica Latinoamericana : Organo De La Asociacion Latinoamericana De Ciencias Fisiologicas Y De La Asociacion Latinoamericana De Farmacologia
|January 1, 1987
Abstract:
A methodology is described to obtain, in frog urinary bladder, isolated epithelial layers suitable for patch clamp studies. This was achieved combining negative pressure, enzymatic digestion, and microdissection. Single channels recordings were obtained from the basolateral membranes while the tissue maintained its general structure and polarity.