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Temperature-induced inactivation of cytoplasmic biogel osmosensing properties is associated with suppression of
Alexandra Platonova1, Francis Boudreault, Leonid V Kapilevich
1Research Centre, Centre hospitalier de l' Université de Montréal (CRCHUM), Montreal, QC, Canada.
The Journal of Membrane Biology
|May 21, 2014
Summary
Cellular heat treatment impairs cytoplasmic gel osmosensing, crucial for cell volume regulation during hypotonic swelling. This indicates the biogel
Area of Science:
- Cell Biology
- Biophysics
- Physiology
Background:
- Intracellular signaling in cell volume regulation is not fully understood.
- Cytoplasmic gel plays a role in cell volume changes, as evidenced by higher volume changes in permeabilized cells compared to intact cells.
Purpose of the Study:
- To investigate the role of cytoplasmic biogel in cell volume regulation.
- To examine the effect of heat treatment on volume changes in intact and permeabilized A549 cells.
Main Methods:
- A549 cells were subjected to short-term heat treatment (48°C and 50°C).
- Cell volume changes were measured in response to osmotic challenges (hypotonic and hyperosmotic conditions).
- The activity of potassium channels was assessed.
Main Results:
- Heat treatment suppressed swelling induced by osmotic changes in both intact and permeabilized cells.
- Heat treatment abolished the effect of hypotonic medium on permeabilized cell volume, indicating suppression of cytoplasmic osmosensing.
- Heat treatment blocked regulatory volume decrease (RVD) and potassium channel activity during hypotonic swelling.
- Hyperosmotic shrinkage was reduced by heat treatment.
Conclusions:
- Cytoplasmic gel osmosensing is a key event in RVD during hypotonic swelling.
- Heat affects cytoplasmic biogel osmosensing rather than plasma membrane water permeability.
- Further research is needed on the role of biogel and plasma membrane in hyperosmotic signaling.
