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Basic investigations in Saccharomyces cerevisiae.

Brendan P G Curran1, Virginia Bugeja

  • 1School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, E1 4NS, UK, B.curran@qmul.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2014
PubMed
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This guide offers essential yeast cell techniques, covering cultivation, storage, mating, and sporulation for Saccharomyces cerevisiae research. It highlights web resources and genetic crossing methods for post-genomic studies.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Yeast (Saccharomyces cerevisiae) is a model organism in biological research.
  • Post-genomic era research increasingly relies on web-based resources for yeast investigation.
  • Standardized protocols are crucial for reproducible yeast cell manipulation.

Purpose of the Study:

  • To serve as a comprehensive reference for fundamental yeast cell procedures.
  • To guide researchers in accessing, cultivating, storing, mating, and sporulating yeast.
  • To introduce essential web-based resources for Saccharomyces cerevisiae research.

Main Methods:

  • Detailed recipes for various yeast growth media.
  • Protocols for achieving optimal cell numbers and growth cycle stages.

Related Experiment Videos

  • Methods for both short-term and long-term yeast cell storage.
  • Techniques for setting up genetic crosses, including diploid cell sporulation and spore isolation.

    • Main Results:

    • Provides accessible information on yeast cell culture and maintenance.
    • Enables researchers to perform genetic crosses and sporulation efficiently.
    • Integrates the use of web-based resources for yeast research.

    Conclusions:

    • This chapter equips researchers with the necessary skills for comprehensive yeast cell management.
    • Facilitates advanced studies in Saccharomyces cerevisiae using established and novel techniques.
    • Serves as a valuable resource for both novice and experienced yeast researchers.