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Serum biomarkers of Keshan disease assessed using a protein profiling approach based on ClinProt technique
YouZhang Xiang1, Qun Xu, WuHong Tan
1Shandong Institute for Endemic Disease Control, Number 11 Yan Dong Xin Road, Jinan, 250014, Shandong, People's Republic of China, xiangyouzhang@163.com.
Insights
Researchers identified distinct protein profiles in serum from Keshan disease (KD) patients, aiding in the differential diagnosis of this endemic myocardiopathy. These protein markers could offer insights for future KD treatment strategies.
Area of Science:
- Cardiology
- Biochemistry
- Proteomics
Background:
- Keshan disease (KD) is an endemic myocardiopathy prevalent in China with an unknown etiology.
- Idiopathic dilated cardiomyopathy (IDCM) shares some clinical similarities, necessitating differential diagnostic markers.
Purpose of the Study:
- To identify differential protein expression in serum from KD patients compared to healthy controls and IDCM patients.
- To discover specific biological markers for the accurate differential diagnosis of KD.
Main Methods:
- Serum samples from 65 KD patients, 29 IDCM patients, 62 controls from KD endemic areas, and 28 controls from non-KD areas were analyzed.
- ClinProt coupled with MALDI-ToF mass spectrometry was employed for protein profiling.
- Bioinformatics algorithms including genetic algorithm, quick classifier, and supervised neural network were used for marker screening and diagnostic model development.
Main Results:
- Thirty-four differential protein peaks distinguished KD patients from healthy controls in non-KD areas.
- Thirty-eight differential peaks were found between KD patients and controls from KD areas.
- Sixty-seven differential peaks were identified when comparing KD patients with IDCM patients.
Conclusions:
- The identified differential protein peaks in KD patients, healthy controls, and IDCM patients show potential as biomarkers.
- These protein signatures may provide valuable clues for the differential diagnosis and subsequent treatment of Keshan disease.
Abstract:
The etiology of Keshan disease (KD), an endemic myocardiopathy in regions of China, is largely unknown. To show the protein changes in serum from KD patients versus controls and idiopathic dilated cardiomyopathy (IDCM) and to search specific biological markers for differential diagnosis for KD. Serum of 65 patients with KD was compared with 29 patients with IDCM, 62 controls from KD areas and 28 controls from non-KD areas by ClinProt/MALDI-ToF technique. The genetic algorithm, quick classifier algorithm and supervised neural network algorithm methods were used to screen marker proteins and establish diagnostic model. Thirty-four differential peaks were identified in KD patients compared with the healthy controls from non-KD areas. Thirty-eight differentially peaks were identified in KD patients and controls from KD areas; and sixty-seven differentially peaks were identified in patients with KD and patients with IDCM. We believe that marker protein peaks screened in KD patients, healthy controls and IDCM patients may provide clues for the differential diagnosis and treatment of KD.