Related Experiment Videos
Isolation and functional characterization of DNA damage repair protein (DRT) from Lepidium latifolium L
Vimlendu Bhushan Sinha1, Atul Grover2, Zakwan Ahmed2
1Defence Institute of Bio-Energy Research, Goraparao, PO Arjunpur, Haldwani 263139, India; Department of Biotechnology, Kumaun University, Bhimtal Campus, Uttarakhand 263136, India; University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka Sec. 16C, New Delhi 110078, India.
Abstract:
We have isolated and in silico characterized a cold regulated plastocyanin encoding gene from Lepidium latifolium L designated as LlaDRT. Its cDNA sequence (JN214346) consists of a 504 bp ORF, 48 and 205 bp of 5' and 3' UTR regions, respectively encoding a protein of 17.07 KDa and pI 4.95. In silico and phylogenetic analysis of LlaDRT suggested that the protein has features of a typical plastocyanin family member and of a nearest relative of the predominant isoform of Arabidopsis (PETE2) plastocyanin. Validation of stress response of LlaDRT by qPCR under different abiotic stress regulators viz salicylic acid, jasmonic acid, calcium chloride, ethylene and abscisic acid revealed its possible regulation and crosstalk amongst different pathways.