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Investigating angiotensin II concentration in modulating Plasmodium falciparum infection and angiotensin receptor
Auley De1, Aparna Tiwari1,2, Medha Agarwal1
1Department of Epidemiology, Indian Council of Medical Research (ICMR)-National Institute of Malaria Research, New Delhi, India.
Introduction:
Angiotensin II (Ang II) has been proposed to protect from severe malaria by killing the parasite and maintaining the blood-brain barrier integrity by acting through type 1 (AT1R) and type 2 (AT2R) receptors. However, this protection from severe malaria is counterproductive in adulthood as the elevated plasma Ang II puts the individual at risk for developing hypertension. Various natural and synthetic peptides of Ang II have been explored to check its antiplasmodial actions despite Ang II being a significant vasopressor via its actions through AT1R. Therefore, to be able to explore Ang II as a potential antiplasmodial agent, it is important to demonstrate its IC50 against P. falciparum in vitro and its minimum effective concentration required for activation of AT1R. It is desirable that IC50 of Ang II against P. falciparum remains below the threshold for AT1R activation and hence to verify this, the current study was planned as there are no previously published reports for determining the threshold Ang II concentration for AT1R activation.
Methods:
For invasion inhibition assay, Pf-infected RBCs (schizonts) were incubated with four different concentrations of Ang II for 16 hours. The activation of AT1R and AT2R in the presence of Ang II (from 10-9 M to 10-7 M) was tested by calcium and cGMP signaling assays, respectively using the human brain microvascular endothelial cell line as an in vitro model.
Results:
Effective Ang II concentration among all tested concentrations was found to be 10-6 M against Plasmodium falciparum invasion. Statistically non-significant and negligible responses in AT1R and AT2R activation were observed below the desired Ang II concentration of 10-6 M.
Discussion:
The results indicate that the IC50 of Ang II against P. falciparum is below the threshold for AT1R activation. However, the minimum effective Ang II concentration required for AT1R activation remains unanswered which demands further improvement in experimental designs.
