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Related Experiment Videos

Selenium in platelets.

J Kiem1

  • 1Institute of Medicine, Nuclear Research Center Jülich GmbH, F.R.G.

Biological Trace Element Research
|January 1, 1988
PubMed
Summary

Human platelets offer a more sensitive indicator of selenium deficiency than plasma. Improved analytical methods reveal narrower normal ranges for platelet selenium, aiding in the detection of latent selenium deficiency states.

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Area of Science:

  • Biochemistry
  • Hematology
  • Nutritional Science

Background:

  • Blood components are routinely analyzed for selenium deficiency detection.
  • Previous studies reported selenium concentrations in human platelets.
  • Analytical methods for elemental analysis have seen significant improvements.

Purpose of the Study:

  • To investigate if human platelets provide superior or additional information for detecting selenium deficiency compared to plasma.
  • To refine analytical methods for precise elemental analysis in platelets.
  • To establish reliable normal ranges for selenium and other mineral elements in platelets.

Main Methods:

  • Utilized neutron activation analysis (NAA) and atomic absorption spectrophotometry (AAS) for elemental analysis.
  • Implemented precise controls for thermal neutron flux and (n, gamma)-cross sections.
  • Accounted for mean platelet wet weight and water fraction in measurements.
  • Reproducibly measured selenium concentrations in platelets.

Main Results:

  • Achieved narrow normal ranges for selenium and other mineral elements in human platelets.
  • Reproduced previously reported selenium concentrations in platelets (approx. 500 ng/g).
  • Observed narrower normal ranges for platelet selenium compared to plasma selenium.
  • Found that platelet selenium levels did not consistently correlate with plasma selenium levels.

Conclusions:

  • Human platelets serve as a valuable indicator for detecting selenium deficiency.
  • Platelet selenium analysis offers advantages over plasma analysis, especially for identifying latent deficiency states.
  • Improved analytical techniques enhance the diagnostic utility of cellular components in nutritional status assessment.

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