Electrospray differential mobility analysis for nanoscale medicinal and pharmaceutical applications

Area of Science:

• Nanotechnology
• Analytical Chemistry
• Biomedical Engineering

Background:

• Electrospray differential mobility analysis (ES-DMA) is an emerging nanotechnology tool.
• It provides label-free multimodal size distributions for proteins and particles (1-500 nm) with subnanometer precision.
• ES-DMA has potential clinical utility in detecting and quantifying lipoproteins, glycoproteins, viruses, amyloids, and bacterial infections.

Purpose of the Study:

• To critically review the contributions of ES-DMA to medicine, pharmaceutical practice, and production.
• To evaluate ES-DMA's use in diagnostic strategies.
• To focus on ES-DMA's role in treatment strategies and process analytical technology (PAT).

Main Methods:

• Critical review of existing literature on ES-DMA applications.
• Evaluation of ES-DMA's performance in diagnostic contexts (lipoproteins, infections, viruses, amyloidosis).
• Assessment of ES-DMA's utility in therapeutic applications (vaccines, gene delivery) and process monitoring.

Main Results:

• ES-DMA enables rapid, label-free characterization of nanoscale entities.
• It has demonstrated utility in detecting and quantifying various biomarkers and pathogens.
• ES-DMA contributes to developing advanced therapies like virus-like particle vaccines and nanoparticle gene vectors.

Conclusions:

• ES-DMA is a powerful tool for nanoscale characterization with broad applications in medicine and pharmaceuticals.
• Its capabilities support diagnostic advancements, novel therapeutic strategies, and process optimization.
• ES-DMA shows significant promise as a process analytical technology (PAT) for pharmaceutical production.