Development of a paper-type tyrosinase biosensor for detection of phenolic compounds
Özge Şenyurt1, Füsun Eyidoğan, Remziye Yılmaz
1Department of Biological Sciences, Nanobiotechnology R&D Lab, Middle East Technical University, Ankara, Turkey; Nanobiz Ltd, METU-Technopolis, Ankara, Turkey.
Abstract:
A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.
