Related Experiment Video
Updated: Apr 29, 2026
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
Published on: February 27, 2016
A succinyl lysine-based photo-cross-linking peptide probe for Sirtuin 5
Karunakaran A Kalesh1, Edward W Tate
1Department of Chemistry, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK. e.tate@imperial.ac.uk.
None:
A succinylation-specific photo-cross-linking peptide probe has been developed for the NAD(+)-dependent hydrolase Sirtuin 5. The probe, not only displayed robust labelling performance with purified Sirt5, but also enabled sensitive detection of the hydrolase in the presence of large excess of cellular proteins. It is anticipated that this probe, and future generations of it, will provide useful chemical tools for the functional analysis of Sirt5 and for the recently discovered PTM of lysine succinylation.
More Related Videos
10:12Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
Published on: April 21, 2023
12:11Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
