Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

Atomic Emission Spectroscopy: Instrumentation

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.

Pulse Oximetry

Pulse Oximetry

Pulse oximetry, or SpO2, is a non-invasive method for continuously monitoring arterial oxygen saturation (SaO2). This procedure involves attaching a probe or sensor to the patient's fingertip, forehead, earlobe, or nose bridge. The sensor works by detecting changes in oxygen saturation levels through light signals generated by the oximeter and reflected by the pulsing blood under the probe.
Purpose
Average SpO2 values are greater than 95%. If the readings fall below 90%, it indicates that...

Atomic Absorption Spectroscopy: Instrumentation

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...

Oxygenic Photosynthesis

Oxygenic Photosynthesis

Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intermittent parathyroid hormone employs autonomous and non-autonomous mechanisms to drive osteogenesis from Ebf3-expressing skeletal progenitor cells.

bioRxiv : the preprint server for biology·2026

Same author

ECgo: All-optical induction of single endothelial cell injury and capillary occlusion in the brain.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Intrinsic endothelial remodeling drives brain capillary repair.

Neuron·2026

Same author

Differential effects of phenylephrine on brain versus skeletal muscle microvascular perfusion: an in vivo laboratory study using translational rodent models.

Canadian journal of anaesthesia = Journal canadien d'anesthesie·2026

Same author

Towards noninvasive white blood cell count.

Biomedical optics express·2026

Same author

Mismatch between oxygen delivery and consumption in the cerebral watershed during subacute global hypoperfusion.

Experimental neurology·2026

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Two-photon antenna-core oxygen probe with enhanced performance.

Emmanuel Roussakis¹, Joel A Spencer, Charles P Lin

¹Department of Biochemistry and Biophysics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.

Analytical Chemistry

|May 23, 2014

Summary

This summary is machine-generated.

A new phosphorescent probe, PtTCHP-C307, enhances two-photon phosphorescence lifetime microscopy (2PLM) for noninvasive oxygen measurements. This improved probe offers higher signal output and sensitivity for in vivo tissue oxygenation studies.

More Related Videos

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Published on: May 4, 2011

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Related Experiment Videos

Last Updated: Apr 29, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Published on: May 4, 2011

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Area of Science:

Biomedical Optics
Molecular Imaging
Physiological Monitoring

Background:

Two-photon phosphorescence lifetime microscopy (2PLM) enables noninvasive 3D oxygen measurements in vivo.
Current phosphorescent probes have limitations including low quantum yield, inefficient energy transfer, and oxygen sensitivity issues.

Purpose of the Study:

To develop a novel phosphorescent probe (PtTCHP-C307) to overcome limitations of existing probes for 2PLM.
To enhance signal output, energy transfer efficiency, and oxygen sensitivity for improved 2PLM performance.

Main Methods:

Design and synthesis of a new antenna-core phosphorescent probe, PtTCHP-C307.
Characterization of probe properties including phosphorescence quantum yield and energy transfer efficiency.
In vivo validation of the probe for oxygen tension (pO2) measurements in mouse bone marrow through the skull.

Main Results:

PtTCHP-C307 exhibits significantly increased phosphorescence quantum yield and antenna-to-core energy transfer efficiency.
The new probe shows minimized phosphorescence quenching and an increased signal dynamic range.
PtTCHP-C307 provides up to a 6-fold higher signal output compared to previous probes under identical excitation.

Conclusions:

PtTCHP-C307 represents a significant advancement in phosphorescent probe design for 2PLM.
The enhanced probe performance enables more sensitive and robust in vivo oxygen measurements.
This technology has potential applications in studying oxygenation in challenging biological environments like bone marrow.