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Related Concept Videos

Hand hygiene01:23

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Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
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The relative amount of a given solution component is known as its concentration. Often, though not always, a solution contains one component with a concentration that is significantly greater than that of all other components. This component is called the solvent and may be viewed as the medium in which the other components are dispersed or dissolved. Solutions in which water is the solvent are, of course, very common on our planet. A solution in which water is the solvent is called an aqueous...
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A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
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Diluting chlorhexidine gluconate: one scrub or two?

Tarun Inder Bajaj1, Cecilia Loh, David Borgstrom

  • 1Department of Surgery, Bassett Medical Center , Cooperstown, New York.

Surgical Infections
|May 23, 2014
PubMed
Summary
This summary is machine-generated.

Diluting two percent chlorhexidine gluconate (CHG) does not affect its antiseptic efficacy. Two scrubs are more effective than one for reducing skin bacteria.

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Area of Science:

  • Antimicrobial efficacy
  • Surgical site infection prevention
  • Skin antisepsis

Background:

  • Two percent chlorhexidine gluconate (CHG) is a common skin antiseptic for surgical patients.
  • CHG is often diluted with saline in practice, but its efficacy when diluted is unknown.
  • Manufacturer guidelines recommend two scrubs, but the effectiveness of single versus double scrubbing is not well-established.

Purpose of the Study:

  • To determine if diluting CHG affects its antiseptic potency.
  • To compare the efficacy of one versus two CHG scrubs.

Main Methods:

  • Healthy subjects were randomized into groups receiving different CHG dilutions (20%-100%) or saline control.
  • Colony-forming units (CFU) were counted before and after skin preparation.
  • A second study compared one versus two CHG scrubs on subjects, with CFU counts taken after each application.

Main Results:

  • All CHG dilution groups showed nearly 100% CFU reduction, significantly greater than the saline control (80% reduction).
  • Diluting CHG to concentrations as low as 0.4% did not compromise its antiseptic efficacy.
  • Two CHG scrubs resulted in significantly lower CFU counts compared to a single scrub.

Conclusions:

  • CHG can be diluted without compromising its antiseptic effectiveness.
  • Two CHG skin scrubs are superior to a single scrub for reducing bacterial counts.
  • Findings support the use of diluted CHG and reinforce the recommendation for two scrubs in surgical preparation.