Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of reverse transcriptase genes in Fulvia fulva.

M T McHale1, I N Roberts, N J Talbot

  • 1Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, Norfolk, England.

Molecular Plant-Microbe Interactions : MPMI
|July 1, 1989
PubMed
Summary

The fungal pathogen Fulvia fulva possesses a reverse transcriptase (RNA-dependent DNA polymerase) gene, potentially explaining its rapid evolution of new races. This finding marks the first detection of reverse transcriptase in filamentous fungi.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ribosomal DNA variation and population differentiation in Salicornia L.

The New phytologist·2021
Same author

Targeting Fungicide Inputs According to Need.

Annual review of phytopathology·2017
Same author

Assessment of the Virulence Spectrum and Its Association with Genetic Diversity in Magnaporthe oryzae Populations from Sub-Saharan Africa.

Phytopathology·2017
Same author

Avirulence (AVR) Gene-Based Diagnosis Complements Existing Pathogen Surveillance Tools for Effective Deployment of Resistance (R) Genes Against Rice Blast Disease.

Phytopathology·2017
Same author

Tibiotalocalcaneal arthrodesis with a compressive retrograde nail: A retrospective study of 59 nails.

Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons·2015
Same author

Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici.

Fungal genetics and biology : FG & B·2015

Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • The fungal pathogen Fulvia fulva is known to rapidly evolve new races.
  • Reverse transcriptase (RNA-dependent DNA polymerase) is crucial for retroviral replication and mobile genetic elements.

Purpose of the Study:

  • To investigate the genetic mechanisms underlying the rapid evolution of fungal pathogens.
  • To determine if Fulvia fulva expresses reverse transcriptase.

Main Methods:

  • Genomic library construction and screening (lambda gt11).
  • DNA sequencing and homology analysis.
  • Southern and Western blot analyses.

Main Results:

  • A clone (P5) with high sequence homology to a Drosophila retrotransposon reverse transcriptase gene was identified in F. fulva.

Related Experiment Videos

  • P5-related sequences are moderately reiterated in the F. fulva genome, with some exhibiting polymorphisms across different races.
  • Immunoreactive proteins to reverse transcriptase antibodies were detected in F. fulva extracts.

    • Conclusions:

    • Fulvia fulva expresses genes for reverse transcriptase, a novel finding in filamentous fungi.
    • The presence of reverse transcriptase may contribute to the pathogen's high mutation rate and rapid adaptation.
    • This discovery opens avenues for understanding fungal evolution and pathogenicity.