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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
Published on: January 19, 2011
Two-dimensional fluorescence-detected coherent spectroscopy with absolute phasing by confocal imaging of a dynamic
Arijit K De1, Daniele Monahan2, Jahan M Dawlaty2
1Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.
Abstract:
We present a novel experimental scheme for two-dimensional fluorescence-detected coherent spectroscopy (2D-FDCS) using a non-collinear beam geometry with the aid of "confocal imaging" of dynamic (population) grating and 27-step phase-cycling to extract the signal. This arrangement obviates the need for distinct experimental designs for previously developed transmission detected non-collinear two-dimensional coherent spectroscopy (2D-CS) and collinear 2D-FDCS. We also describe a novel method for absolute phasing of the 2D spectrum. We apply this method to record 2D spectra of a fluorescent dye in solution at room temperature and observe "spectral diffusion."
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