Related Experiment Video
Updated: Apr 29, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
Transforming growth factor-Beta inhibits heme oxygenase-1 expression in lung fibroblast through nuclear
Ying Xie1, Ye Wang, Chuanyue Zong
1Department of Anesthesiology, Huai'an Hospital Affiliated with Xuzhou Medical College and Huai'an Second People's Hospital, Huaian, China.
Background:
Heme oxygenase-1 (HO-1) contributes to the pathogenesis of pulmonary fibrosis. However, the expression of HO-1 in fibroblasts under fibrotic conditions has not been studied.
Methods:
This study was conducted to investigate the expression of HO-1 in lung fibroblasts from mice and humans under fibrotic conditions by Western blot.
Results:
We found that the expression of HO-1 was significantly decreased in lung fibroblasts isolated from bleomycin-challenged mice in comparison with control mice. Transforming growth factor-β (TGF-β) inhibited HO-1 expression and induced differentiation in human lung fibroblasts. Pretreatment with nuclear factor-κB (NF-κB) activation inhibitor or knockdown of the NF-κB p65 subunit attenuated TGF-β-induced inhibition of HO-1 expression and differentiation in human lung fibroblasts. Similarly, lysophosphatidic acid (LPA) induced TGF-β expression and decreased HO-1 expression in human lung fibroblasts. Interestingly, pretreatment with neutralized anti-TGF-β antibody attenuated LPA effects in human lung fibroblasts.
Conclusion:
These data suggested that TGF-β inhibited HO-1 expression in human lung fibroblasts through activation of NF-κB.
More Related Videos
06:02Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
Published on: June 10, 2016
07:49Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
Related Concept Videos
TGF - β Signaling Pathway
Regulation of Angiogenesis and Blood Supply
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
General Transcription Factors
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
Transcription Factors