Related Experiment Video
Updated: Apr 29, 2026
09:53
Isolating Mesangiogenic Progenitor Cells MPCs from Human Bone Marrow
Published on: July 15, 2016
9.9K
A SAGE View of Mesenchymal Stem Cells
1Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA, USA.
International Journal of Stem Cells
|May 24, 2014
Summary
Mesenchymal stem cells (MSCs) are more diverse than previously thought, with distinct subpopulations driving their therapeutic effects. This functional heterogeneity is key to MSCs
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Immunology
Background:
- Mesenchymal stem cells (MSCs) were initially defined by their differentiation potential and role in hematopoiesis.
- Recent studies highlight MSC therapeutic efficacy in various diseases, mediated by paracrine signaling.
- This contrasts with the traditional definition of MSCs as rare, hierarchical progenitor cells.
Purpose of the Study:
- To investigate the heterogeneity of MSC populations.
- To understand the functional basis of MSC therapeutic effects.
Main Methods:
- Analysis of the MSC transcriptome using Serial Analysis of Gene Expression (SAGE).
- Identification of distinct subpopulations expressing different regulatory proteins.
Main Results:
- MSC populations exhibit significant heterogeneity.
- MSCs express proteins involved in mesoderm specification, angiogenesis, hematopoiesis, cell communication, and neural activities.
- Different regulatory proteins are expressed in distinct MSC subpopulations.
Conclusions:
- MSC populations are functionally heterogeneous.
- This heterogeneity significantly contributes to the therapeutic effects of MSCs in various conditions.
Related Concept Videos
01:19Mesenchymal Stem Cells
4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K
01:21Satellite Stem Cells and Muscular Dystrophy
1.7K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
1.7K
01:27Source And Potency Of Stem Cells
5.0K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
5.0K
01:33Adult Stem Cells
27.8K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
27.8K
00:57Embryonic Stem Cells
4.5K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.5K
00:58Embryonic Stem Cells
25.8K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
25.8K