Assessing regulated nuclear transport in Saccharomyces cerevisiae

Regulated protein transport between the cytoplasm and the nucleoplasm occurs through nuclear pore complexes and is critical to the function of numerous biological pathways. Saccharomyces cerevisiae has been used as a model system to probe the underlying mechanisms of nuclear transport and how they regulate various physiological processes. This has been facilitated, in part, by studies that couple the microscopic observation of a fluorescently tagged transport cargo's in vivo localization with numerous genetic and biochemical tools available to yeast researchers. Here, we describe some of these methods as they pertain to studies on regulated nuclear transport.