Related Experiment Video
Updated: Apr 29, 2026

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
Published on: December 5, 2025
Bioactive secondary metabolites from Phomopsis sp., an endophytic fungus from Senna spectabilis
Vanessa Mara Chapla1, Maria Luiza Zeraik2, Valdecir F Ximenes3
1NuBBE - Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Departamento de Química Orgânica, Instituto de Química, UNESP, Universidade Estadual Paulista, Araraquara-SP, 14800-900, Brazil. vanessachapla@gmail.com.
Abstract:
Chemical investigation of an acetonitrile fraction from the endophytic fungus Phomopsis sp. led to the isolation of the new natural product 2-hydroxy-alternariol (7) together with the known compounds cytochalasins J (1) and H (2), 5'-epialtenuene (3) and the mycotoxins alternariol monomethyl ether (AME, 4), alternariol (AOH, 5) and cytosporone C (6). The structure of the new compound was elucidated by using 1-D and 2-D NMR (nuclear magnetic resonance) and high resolution mass spectrometry. The cytochalasins J (1) and H (2) and AOH (5) exhibited potent inhibition of the total ROS (reactive oxygen species) produced by stimulated human neutrophils and acted as potent potential anti-inflammatory agents. Moreover, cytochalasin H (2) demonstrated antifungal and acetylcholinesterase enzyme (AChE) inhibition in vitro.
Related Concept Videos
Fungal Phylum Basidiomycota
Microbe-Plant Interactions
Antifungal Agents
Bacterial Phylum Planctomycetes
Fungal Phylum Ascomycota
Production of Antibiotics

