Related Experiment Video
Updated: Apr 29, 2026
06:32
Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions
Published on: June 13, 2025
680
Shiga toxin 2f-producing Escherichia albertii from a symptomatic human
Koichi Murakami1, Yoshiki Etoh, Eri Tanaka
1Fukuoka Institute of Health and Environmental Sciences.
Japanese Journal of Infectious Diseases
|May 27, 2014
Summary
A Shiga toxin-producing bacterium, previously identified as E. coli, is now classified as E. albertii. This reclassification is crucial for public health, as E. albertii can cause human diarrhea.
Area of Science:
- Microbiology
- Bacteriology
- Public Health
Background:
- A Shiga toxin (Stx) 2f-producing Escherichia coli O115:HNM strain (F08/101-31) was isolated from a symptomatic human.
- This strain was initially misidentified as E. coli due to atypical biochemical characteristics for the species E. albertii.
Purpose of the Study:
- To reclassify the bacterial strain F08/101-31 using advanced genomic and typing methods.
- To assess the public health implications of misidentifying E. albertii as E. coli, particularly regarding Shiga toxin production and human illness.
Main Methods:
- Whole genome DNA-DNA hybridizations
- Gene sequencing (cpn60, dnaJ, 16S rRNA)
- Multi-locus sequence typing (MLST)
Main Results:
- The strain F08/101-31 was confirmed to be E. albertii, not E. coli.
- The study identified that some E. albertii strains produce Shiga toxins and are associated with human diarrheal illness.
- Misidentification of E. albertii as E. coli is a significant issue in Japan.
Conclusions:
- The reclassification of F08/101-31 as E. albertii necessitates a review of diagnostic and management strategies.
- Updated guidelines are required in Japan for the identification and control of Shiga toxin-producing E. albertii to prevent public health risks.
More Related Videos
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
2.6K
06:20Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Published on: April 9, 2018
8.8K
Related Concept Videos
01:18Bacterial Gastroenteritis
88
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
88
01:25Cholera
147
Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...
147
01:12Bacterial Toxins
139
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
139
01:23Stringent Response in E. coli
526
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
526
01:30Reservoir of Infection
88
Infectious diseases arise from intricate interactions between pathogens and their reservoirs. A reservoir of infection refers to the natural habitat where a pathogen lives, grows, and multiplies, serving as a continual source of infection. Reservoirs are broadly classified as either living or nonliving, and each plays a unique role in disease transmission, significantly influencing public health interventions and control strategies.Humans act as reservoirs for a wide array of pathogens,...
88