Related Experiment Video

Updated: Apr 29, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

5.6K

Glucocorticoids induce apoptosis by inhibiting microRNA cluster miR‑17‑92 expression in chondrocytic cells

Wenhua Xing1, Lixia Hao2, Xuejun Yang1

  • 1Department of Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical University, Huimin, Hohhot, Inner Mongolia Autonomous Region 010058, P.R. China.

Molecular Medicine Reports
|May 27, 2014
PubMed

Insights

Glucocorticoids (GCs) induce chondrocyte apoptosis by inhibiting the microRNA-17-92 (miR-17-92) cluster. This suggests microRNAs play a key role in GC-induced skeletal development impairment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Sustained glucocorticoid (GC) treatment impairs skeletal development.
  • The specific effects of GCs on chondrocytes, crucial for skeletal development, are under-investigated.
  • Understanding GC-induced chondrocyte apoptosis is vital for skeletal health.

Purpose of the Study:

  • To investigate the role of the microRNA-17-92 (miR-17-92) cluster in GC-induced chondrocyte apoptosis.
  • To utilize HCS-2/8 cells as an in vitro model for human chondrocytes.
  • To elucidate the molecular mechanisms underlying GC effects on chondrocytes.

Main Methods:

  • Utilized HCS-2/8 cells as an in vitro model of human chondrocytes.
  • Administered dexamethasone (Dex) to induce apoptosis.
  • Assessed apoptosis-associated molecule expression.
  • Measured the expression levels of the miR-17-92 cluster.

Main Results:

  • Dexamethasone (Dex) induced apoptosis in HCS-2/8 chondrocytic cells.
  • Dex treatment led to high expression of apoptosis-associated molecules.
  • Expression of the miR-17-92 cluster was significantly inhibited during Dex-induced apoptosis.

Conclusions:

  • Inhibition of the miR-17-92 cluster contributes to Dex-induced apoptosis in chondrocytes.
  • MicroRNAs are implicated in glucocorticoid-induced impairment of chondrocytes.
  • The miR-17-92 cluster may serve as a potential therapeutic target for mitigating GC-induced skeletal damage.

More Related Videos

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.6K
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity08:30

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Published on: February 24, 2017

9.0K

Related Concept Videos

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids01:21

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...
691
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.0K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.0K