Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

891
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
891
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.9K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of the disease causal genes based on heterogeneous network and multi-feature combination method.

Computational biology and chemistry·2022
Same author

Room Temperature Phosphorescence Emission From Multi-States.

Frontiers in chemistry·2022
Same author

Unusual Cause of Upper Gastrointestinal Hemorrhage.

Gastroenterology·2022
Same author

Tanshinone I Mitigates Steroid-Induced Osteonecrosis of the Femoral Head and Activates the Nrf2 Signaling Pathway in Rats.

Evidence-based complementary and alternative medicine : eCAM·2022
Same author

MAL protein suppresses the metastasis and invasion of GC cells by interfering with the phosphorylation of STAT3.

Journal of translational medicine·2022
Same author

Oncogene or Tumor Suppressor: The Coordinative Role of Lysine Methyltransferase SET7/9 in Cancer Development and the Related Mechanisms.

Journal of Cancer·2022
Same journal

SVM-Based Optical Detection of Retinal Ganglion Cell Apoptosis.

Photonics·2026
Same journal

The Cone Optoretinogram as a Function of Retinal Eccentricity.

Photonics·2026
Same journal

Influence of Uncertainties in Optode Positions on Self-Calibrating or Dual-Slope Diffuse Optical Measurements.

Photonics·2025
Same journal

Air Core ARROW Waveguides Fabricated in a Membrane-Covered Trench.

Photonics·2025
Same journal

In Situ Structural Characterization of Cardiomyocyte Microenvironment by Multimodal STED Microscopy.

Photonics·2024
Same journal

Adjunct Nondamaging Focal Laser Reduces Intravitreal Injection Burden in Diabetic Macular Edema.

Photonics·2024
See all related articles

Related Experiment Video

Updated: Apr 29, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.2K

Instrumentation in Diffuse Optical Imaging.

Xiaofeng Zhang1

  • 1Department of Radiology, Duke University Medical Center, DUMC 3808, Durham, NC 27710, USA.

Photonics
|May 27, 2014
PubMed
Summary
This summary is machine-generated.

Diffuse optical imaging utilizes light propagation in biological tissues for diverse medical and biological applications. This review covers recent advancements in instrumentation and methods for enhanced imaging contrast and accuracy.

Keywords:
bioluminescenceclinicaldiffuse optical imagingfluorescenceinstrumentationmolecular imagingpreclinicaltomography

More Related Videos

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

18.1K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

4.0K

Related Experiment Videos

Last Updated: Apr 29, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.2K
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

18.1K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

4.0K

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Biophotonics

Background:

  • Diffuse optical imaging (DOI) is a versatile technique with broad applications in biology and medicine.
  • It encompasses various modalities like diffuse optical tomography and bioluminescence imaging.
  • All DOI methods rely on the principle of light propagation through diffusive biological tissues.

Purpose of the Study:

  • To review the latest developments in diffuse optical imaging instrumentation and methodology.
  • To provide a comprehensive overview of system architecture, light sources, and detection techniques.
  • To highlight advancements in spectral separation, signal modulation, and imaging contrast.

Main Methods:

  • Review of recent literature on diffuse optical imaging systems.
  • Analysis of instrumentation components including light sources and photo-detectors.
  • Examination of signal processing and spectral separation techniques.

Main Results:

  • Significant advancements in system configurations and imaging methodologies for DOI.
  • Improved performance in light source, photo-detection, and spectral separation technologies.
  • Enhanced capabilities in signal modulation and achieving higher imaging contrast.

Conclusions:

  • Diffuse optical imaging continues to evolve with rapid advancements in instrumentation and methodology.
  • These developments promise more precise and versatile applications in biological and medical fields.
  • Further innovation in DOI systems will enhance diagnostic and research capabilities.