Dried saliva spot as a sampling technique for saliva samples.

Area of Science:

• Analytical Chemistry
• Biomedical Diagnostics
• Forensic Science

Background:

• Traditional saliva sample collection can be cumbersome and prone to degradation.
• Development of simplified, stable saliva sampling techniques is crucial for various analyses.
• Dried Saliva Spot (DSS) offers a potential alternative for improved sample handling.

Purpose of the Study:

• To evaluate Dried Saliva Spot (DSS) as a novel sampling technique for saliva analysis.
• To determine the local anesthetic lidocaine in DSS using liquid chromatography and mass spectrometry.
• To compare the efficacy of DSS with a previous microextraction by packed sorbent syringe method.

Main Methods:

• Collected 50 μL of saliva on filter paper for the DSS technique.
• Extracted saliva from the filter paper using an organic solvent.
• Quantified lidocaine in DSS using liquid chromatography and mass spectrometry (LC-MS).

Main Results:

• Successfully determined lidocaine in saliva samples collected using the DSS technique.
• Results for lidocaine determination in DSS showed good agreement with previous microextraction methods.
• The DSS method demonstrated ease and efficiency in saliva sampling and extraction procedures.

Conclusions:

• Dried Saliva Spot (DSS) is a viable and effective sampling technique for saliva analysis.
• DSS simplifies saliva sampling, storage, and transport, offering a new dimension in sample handling.
• This method holds promise for routine application in clinical, forensic, and research settings.