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[Comparative study of 4 methods for isolating Salmonella from surface waters].

R J Anselmo1, H Barrios, S Viora

  • 1Universidad Nacional de Luján, Argentina.

Revista Argentina De Microbiologia
|July 1, 1989
PubMed
Summary

This study identified Salmonella serotypes in the Lujan River using four detection methods. Modified Rappaport-Vassiliadis broth with pre-enrichment proved most effective for detecting these bacteria in river water.

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Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Bacteriology

Background:

  • The Lujan River flows through an urban area in Buenos Aires, potentially impacting water quality.
  • Salmonella serotypes are significant waterborne pathogens.
  • Assessing microbial contamination in urban rivers is crucial for public health.

Purpose of the Study:

  • To determine the presence and diversity of Salmonella serotypes in the Lujan River.
  • To compare the efficacy of four different Salmonella detection methods.
  • To establish baseline data for future water quality monitoring projects.

Main Methods:

  • A comparative study of four Salmonella detection methods: Tetrathionate-brilliant green, mannitol-selenite, Rappaport 25, and modified Rappaport-Vassiliadis broth.

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  • Samples were pre-enriched in buffered peptone water.
  • Submerged gauze technique used for sampling at three river locations.
  • Analysis of 200 water samples.

    • Main Results:

    • Salmonella was detected in 46% of the analyzed water samples.
    • The combination of modified Rappaport-Vassiliadis enrichment broth and brilliant green agar with 0.25% sodium deoxycholate, following pre-enrichment, yielded the best results.
    • Sixteen distinct Salmonella serotypes were identified, including common types like S. Typhimurium and S. Enteritidis.

    Conclusions:

    • The Lujan River water is contaminated with a variety of Salmonella serotypes.
    • Modified Rappaport-Vassiliadis broth with pre-enrichment is a highly effective method for Salmonella detection in this environment.
    • Findings highlight the need for water quality management in urban river systems.