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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic
Yueting Wu1, Lele Tang1, Linhong Huang1
1Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
Abstract:
In this study, we reported on a low detection limit penicillin biosensor with layer-by-layer (LbL) film containing single-graphene nanosheets (SGNs) preadsorbed with hematein, ionic liquids (ILs) and penicillinase. The penicillinase catalyzes the hydrolysis of penicillin to penicilloic acid, where H(+) is liberated and monitored amperometrically with hematein as a pH indicator. The SGN-hematein/ILs/penicillinase biosensor exhibited excellent performance for penicillin in PBS with a wide range from 1.25×10(-13) to 7.5×10(-3)M, and a low detection limit of 10(-13)M (0.04ppt, S/N≥3). Furthermore, the detection of penicillin concentration in real sample (milk) had acceptable accuracy with the assay system.
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