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Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
Published on: November 5, 2020
TRIM29 as a novel biomarker in pancreatic adenocarcinoma
Hongli Sun1, Xianwei Dai1, Bing Han1
1Department of Biliary and Vascular Surgery, Shengjing Hospital of China Medical University, Heping District, Shenyang 110004, China.
Background And Aim:
Tripartite motif-containing 29 (TRIM29) is structurally a member of the tripartite motif family of proteins and is involved in diverse human cancers. However, its role in pancreatic cancer remains unclear.
Methods:
The expression pattern of TRIM29 in pancreatic ductal adenocarcinoma was assessed by immunocytochemistry. Multivariate logistic regression analysis was used to investigate the association between TRIM29 and clinical characteristics. In vitro analyses by scratch wound healing assay and invasion assays were performed using the pancreatic cancer cell lines.
Results:
Immunohistochemical analysis showed TRIM29 expression in pancreatic cancer tissues was significantly higher (n = 186) than that in matched adjacent nontumor tissues. TRIM29 protein expression was significantly correlated with lymph node metastasis (P = 0.019). Patients with positive TRIM29 expression showed both shorter overall survival and shorter recurrence-free survival than those with negative TRIM29 expression. Multivariate analysis revealed that TRIM29 was an independent factor for pancreatic cancer over survival (HR = 2.180, 95% CI: 1.324-4.198, P = 0.011). In vitro, TRIM29 knockdown resulted in inhibition of pancreatic cancer cell proliferation, migration, and invasion.
Conclusions:
Our results indicate that TRIM29 promotes tumor progression and may be a novel prognostic marker for pancreatic ductal adenocarcinoma.
Insights
Tripartite motif-containing 29 (TRIM29) promotes pancreatic cancer progression. Higher TRIM29 expression correlates with poorer survival and metastasis, suggesting it as a prognostic marker.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Tripartite motif-containing 29 (TRIM29) is implicated in various human cancers.
- The specific role of TRIM29 in pancreatic cancer pathogenesis is not well understood.
Purpose of the Study:
- To investigate the expression pattern and clinical significance of TRIM29 in pancreatic ductal adenocarcinoma.
- To explore the functional role of TRIM29 in pancreatic cancer cell behavior.
Main Methods:
- Immunohistochemistry was used to assess TRIM29 expression in 186 pancreatic cancer tissues and adjacent non-tumor tissues.
- Multivariate logistic regression analyzed the association between TRIM29 expression and clinical characteristics.
- In vitro studies, including scratch wound healing and invasion assays, were conducted on pancreatic cancer cell lines with TRIM29 knockdown.
Main Results:
- TRIM29 expression was significantly elevated in pancreatic cancer tissues compared to non-tumor tissues.
- TRIM29 expression positively correlated with lymph node metastasis and predicted shorter overall and recurrence-free survival.
- TRIM29 was identified as an independent prognostic factor for pancreatic cancer survival (HR = 2.180, P = 0.011).
- In vitro, TRIM29 knockdown inhibited pancreatic cancer cell proliferation, migration, and invasion.
Conclusions:
- TRIM29 significantly promotes pancreatic ductal adenocarcinoma progression.
- TRIM29 may serve as a novel and valuable prognostic biomarker for pancreatic cancer.
