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Updated: Apr 29, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Kinetic study of the binding of triplex-forming oligonucleotides containing partial cationic modifications to
Yoshiyuki Hari1, Shin Ijitsu1, Masaaki Akabane-Nakata1
1Graduate School of Phamaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita 565-0871, Japan.
Abstract:
Several triplex-forming oligonucleotides (TFOs) partially modified with 2'-O-(2-aminoethyl)- or 2'-O-(2-guanidinoethyl)-nucleotides were synthesized and their association rate constants (kon) with double-stranded DNA were estimated by UV spectrophotometry. Introduction of cationic modifications in the 5'-region of the TFOs significantly increased the kon values compared to that of natural TFO, while no enhancement in the rate of triplex DNA formation was observed when the modifications were in the middle and at the 3'-region. The kon value of a TFO with three adjacent cationic modifications at the 5'-region was found to be 3.4 times larger than that of a natural one. These results provide useful information for overcoming the inherent sluggishness of triplex DNA formation.
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