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Related Experiment Video

Updated: Apr 29, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Construction of a collagen-based, split-thickness cornea substitute.

A Acun¹, V Hasirci

¹a Department of Biotechnology , Middle East Technical University (METU) , Ankara 06800 , Turkey.

Journal of Biomaterials Science. Polymer Edition

|May 29, 2014

Summary

Tissue-engineered corneas offer a promising alternative to grafts for treating corneal defects. This study developed a biomimetic scaffold using collagen foam and fibrous mats, successfully supporting cell growth and maintaining transparency.

Keywords:

cornea electrospinning keratocytes split thickness tissue engineering

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Last Updated: Apr 29, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

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Published on: September 12, 2014

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A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

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Published on: October 6, 2019

Area of Science:

Biomaterials Science
Tissue Engineering
Ophthalmology

Background:

Corneal defects often require allografts, which have limitations.
Tissue-engineered corneas offer tunable biomaterials and patient-specific cell integration.
Mimicking native corneal structure is key for successful regeneration.

Purpose of the Study:

To develop a split-thickness, biomimetic corneal substitute.
To evaluate the suitability of the engineered cornea for cell attachment, growth, and function.
To assess the potential of this construct as an alternative to corneal allografts.

Main Methods:

Fabrication of a collagen-chondroitin sulfate foam stromal layer seeded with human corneal keratocytes (HK).
Electrospinning of a collagen type I fibrous mat epithelial layer seeded with retinal pigment epithelium (RPE) cells.
In vitro characterization, cell co-culture, and assessment of extracellular matrix (ECM) deposition and transparency.

Main Results:

The engineered cornea supported human corneal keratocyte (HK) and retinal pigment epithelium (RPE) cell attachment and growth.
Co-culture of HK and RPE cells enhanced extracellular matrix (ECM) deposition compared to single-cell cultures.
The fibrous layer effectively separated cell types while allowing communication, maintaining high transparency (~80%).
The construct demonstrated good suturability after 30 days of co-culture.

Conclusions:

The developed tissue-engineered cornea shows potential as a viable alternative to allografts.
The biomimetic design supports cellular functions and maintains optical properties.
This split-thickness construct is mechanically suitable for surgical implantation.