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Flow-based impedimetric immunosensor for aflatoxin analysis in milk products
Lizy Kanungo1, Gautam Bacher, Sunil Bhand
1Biosensor Laboratory, Department of Chemistry, BITS, Pilani-K. K. Birla Goa Campus, Goa, 403726, India, lizy_kanungo@yahoo.co.in.
Applied Biochemistry and Biotechnology
|May 29, 2014
Summary
A novel impedance-based method detects aflatoxin M1 (AFM1) and M2 (AFM2) in milk products. This label-free technique offers reliable, low-concentration toxin analysis for enhanced food safety.
Area of Science:
- Analytical Chemistry
- Food Science
- Biosensing Technology
Background:
- Aflatoxins M1 (AFM1) and M2 (AFM2) are hazardous milk contaminants.
- Accurate detection methods are crucial for milk product safety.
Purpose of the Study:
- To develop and validate a label-free impedance sensing technique for AFM1 and AFM2 detection in milk.
- To assess the method's performance in real milk product matrices.
Main Methods:
- Utilized a two-electrode setup with silver (Ag) wire electrodes for impedance measurement.
- Employed a flow-based system with microflow pumps for sample and analyte injection.
- Optimized flow rates and investigated matrix effects using Bode plots.
Main Results:
- Achieved good recoveries for AFM1 detection in the range of 1-100 pg/mL.
- Demonstrated the method's ability to handle matrix effects in various milk products.
- Investigated the cross-reactivity influence of AFM2 on AFM1 detection.
Conclusions:
- The developed impedance sensing technique is effective for label-free AFM1 and AFM2 analysis in milk.
- The method shows potential for online monitoring of these toxins in dairy products.
- This approach contributes to improved food safety and quality control in the dairy industry.
