Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Redox Reactions01:24

Redox Reactions

50.9K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
50.9K
Redox Reactions01:27

Redox Reactions

1.2K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.2K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.8K
Necrosis01:16

Necrosis

5.2K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
5.2K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

6.7K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Reorganization of Developmental to Adult Genome Topology Controls the Initiation and Stabilization of the Human Muscle Stem Cell State.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD.

Nature communications·2025
Same author

Tenascin-C from the tissue microenvironment promotes muscle stem cell maintenance and function through Annexin A2.

Communications biology·2025
Same author

Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells.

Stem cell reports·2025
Same author

MYOD represses gene expression from non-E-box motifs.

Genes & development·2025
Same author

Self-amplifying NRF2-EZH2 epigenetic loop converts KRAS-initiated progenitors to invasive pancreatic cancer.

Nature cancer·2025

Related Experiment Video

Updated: Apr 28, 2026

Cellular Redox Profiling Using High-content Microscopy
11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

10.4K

Redox or death: checking on fetal myogenesis.

Lucia Latella1, Pier Lorenzo Puri2

  • 1Laboratory of Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, Italy; Institute of Translational Pharmacology, National Research Council of Italy, Via Fosso del Cavaliere 100, 00133 Rome, Italy.

Developmental Cell
|May 30, 2014
PubMed
Summary

Skeletal muscle development relies on a Pitx2/Pitx3 network that combats oxidative stress. Without this network, fetal muscle experiences DNA damage and cell death, hindering growth.

More Related Videos

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher
08:09

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Published on: August 16, 2024

5.6K
Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

2.1K

Related Experiment Videos

Last Updated: Apr 28, 2026

Cellular Redox Profiling Using High-content Microscopy
11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

10.4K
Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher
08:09

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Published on: August 16, 2024

5.6K
Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

2.1K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Muscle Physiology

Background:

  • Fetal myogenesis is a critical developmental process.
  • Oxidative stress can negatively impact cellular development.
  • The role of specific transcription factors in muscle antioxidant defense was unclear.

Purpose of the Study:

  • To investigate the role of Pitx2/Pitx3 in protecting differentiating skeletal muscle from oxidative stress.
  • To elucidate the molecular mechanisms underlying Pitx2/Pitx3-mediated protection during fetal myogenesis.

Main Methods:

  • Analysis of gene expression patterns in developing skeletal muscle.
  • Investigating the impact of Pitx2/Pitx3 deficiency on oxidative DNA damage.
  • Assessing apoptosis levels in genetically modified models.

Main Results:

  • A network involving Pitx2/Pitx3 and antioxidant enzymes was identified.
  • This network effectively mitigates reactive oxygen species (ROS) during fetal myogenesis.
  • Genetic deficiency of Pitx2/Pitx3 led to significant oxidative DNA damage and apoptosis.

Conclusions:

  • The Pitx2/Pitx3 network is essential for protecting skeletal muscle during development.
  • Disruption of this network results in irreversible oxidative damage and impaired muscle formation.
  • Understanding this pathway is crucial for addressing developmental muscle disorders.