Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Corrigendum to "Nitrogen source and availability associate to mitochondrial respiratory pathways and symbiotic function in lotus japonicus" [J. Plant Physiol. 2025 (314), November 2025, 154606].

Journal of plant physiology·2025

Same author

Understanding the role of environment in associative learning of nicotine-induced place preference conditioning in zebrafish.

Progress in neuro-psychopharmacology & biological psychiatry·2025

Same author

Post-traumatic retinal siderosis, a case report.

Archivos de la Sociedad Espanola de Oftalmologia·2024

Same author

Pulmonary nocardiosis after covid-19 infection: case report and literature review.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2023

Same author

Belatacept-based immunosuppression in practice: A single center experience.

Transplant immunology·2023

Same author

Neutropenia Secondary to SARS-Cov2 Vaccination in Post-Hematopoietic Stem Cell Transplant (HSCT) Patients.

Mediterranean journal of hematology and infectious diseases·2023

Same journal

Scaphocephaly as a Novel Phenotypic Feature of WDR83OS-Related Neurodevelopmental Disorder: A Case Report With a Novel Variant and Literature Review.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same journal

Sales-Training-Inspired Optimization for Deep High-Order Principal Network in Autism Spectrum Disorder Classification.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same journal

Turkish Validity and Reliability Study of the Executive Functioning Scale and the Daily Living Skills Scale in Children and Adolescents.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same journal

Neuropsychiatric Features, ADHD and Cognitive Disengagement Syndrome in Children and Adolescents With Celiac Disease: A Controlled Study.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same journal

The Bright Side of the Spectrum: Self-Limited Infantile Epilepsy-Related SCN8A Gof Variant.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same journal

Domain-Specific Cognitive Vulnerabilities in ADHD: The Impact of Comorbid Specific Learning Disorder on WISC-IV Profiles.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 28, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Polyamine acetylation in the developing and aging mouse brain.

J Ortiz¹, E Giacobini², T Schmidt-Glenewinkel³

¹Yale University School of Medicine, Department of Pharmacology, New Haven, CT 06510 U.S.A.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience

|May 31, 2014

Summary

This summary is machine-generated.

Polyamine acetylation, including putrescine and spermidine, is significantly higher in neonatal mouse brains than adult brains. This acetylation activity decreases with age, suggesting a key role in developing mouse brain metabolism and potentially GABA production.

More Related Videos

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Related Experiment Videos

Last Updated: Apr 28, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Area of Science:

Neuroscience
Biochemistry
Developmental Biology

Background:

Polyamines like putrescine and spermidine are crucial for cellular functions.
Their acetylation is a metabolic process with implications for biological activity.
Understanding polyamine acetylation in the brain is important for developmental studies.

Purpose of the Study:

To investigate the developmental changes in putrescine and spermidine acetylation in the mouse brain.
To determine the role of polyamine acetylation in neonatal brain development.
To explore the potential link between polyamine acetylation and GABA synthesis.

Main Methods:

Analysis of putrescine and spermidine acetylation activity in different brain fractions (nuclei, mitochondria, microsomes, soluble) at various developmental stages.
Comparison of acetylation levels between neonatal and adult mice.
Fractionation of brain homogenates to isolate specific cellular components.

Main Results:

Acetylation of putrescine and spermidine was significantly higher in neonatal mouse brains compared to adult brains (10-fold).
Acetylation activity showed distinct patterns across different brain fractions and developmental time points.
Activity was present in mitochondrial and soluble fractions of neonatal brains but not adult brains.
Acetylation levels declined with age, with some differences between putrescine and spermidine acetylation patterns during development.

Conclusions:

Polyamine acetylation plays a critical role in the metabolic processes of the developing mouse brain.
The presence of acetylation activity in specific neonatal brain fractions suggests a developmental function.
Putrescine acetylation may contribute to GABA levels in the developing brain.