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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Updated: Apr 28, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Er/Tm:fiber laser system for coherent Raman microscopy.

Nicola Coluccelli, Vikas Kumar, Marco Cassinerio

    Optics Letters
    |May 31, 2014
    PubMed
    Summary

    We developed a new fiber laser for coherent Raman microscopy. This system achieves powerful, narrow-band laser pulses for advanced microscopy applications.

    Area of Science:

    • Optics and Photonics
    • Laser Physics
    • Microscopy

    Background:

    • Coherent Raman microscopy offers label-free chemical imaging.
    • High-power, tunable laser sources are crucial for advanced microscopy techniques.

    Purpose of the Study:

    • To present a novel fiber-based hybrid laser system for coherent Raman microscopy.
    • To demonstrate the system's capability for generating specific pump and Stokes pulses.

    Main Methods:

    • Combining an amplified Er:fiber femtosecond oscillator with a Tm:fiber amplifier.
    • Utilizing nonlinear spectral compression to achieve narrow spectral widths.
    • Boosting the power of the 2-μm supercontinuum to 300 mW.

    Main Results:

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  • Achieved sub-20-cm(-1) wide pump and Stokes pulses.
  • Generated pulses with 2500–3300 cm(-1) frequency detuning.
  • Obtained average power at the 100-mW level for the generated pulses.

    • Conclusions:

    • The developed fiber laser system is suitable for coherent Raman microscopy.
    • The system provides high-power, spectrally tailored pulses for stimulated Raman scattering microscopy.