Facile synthesis of multilayered polysaccharidic vesicles

Area of Science:

• Biomaterials Science
• Nanotechnology
• Drug Delivery Systems

Background:

• Polysaccharides offer biocompatibility and biodegradability for advanced drug carriers.
• Developing efficient drug delivery systems is crucial for targeted therapy and reduced side effects.

Purpose of the Study:

• To develop facile synthesis methods for multilayered polysaccharidic vesicles (mPSVs).
• To investigate the potential of mPSVs for targeted drug delivery and tumor inhibition.

Main Methods:

• Synthesized mPSVs using polysaccharides like starch, hyaluronate (HA), and glycol chitosan (GC) via simple chemistry and enzymatic reactions.
• Utilized enzymatic degradation of the HA shell by hyaluronidase (HYAL) to control drug release.
• Incorporated folate ligand and D-(KLAKLAK)2 peptide for active targeting and apoptosis induction.

Main Results:

• Enzymatic degradation of the HA shell accelerated protein/peptide release from mPSVs.
• mPSVs demonstrated effective accumulation in KB tumor cells through passive targeting (EPR effect) and active targeting (folate receptor binding).
• Significant increase in in vivo tumor inhibition was observed with the mPSV system.

Conclusions:

• The developed mPSVs represent a promising platform for biomedical applications.
• This vesicle system shows potential for delivering small molecular drugs targeting proteins and genes.
• The facile synthesis and targeted delivery capabilities highlight the advancement in polysaccharide-based drug delivery technology.