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Modelling cortical cataractogenesis. X. Evaluation of lens optical function by computer based image analysis using in
K P Mitton1, T Dzialoszynski, J Weerheim
1Department of Biochemistry, University of Western Ontario, London.
Lens and Eye Toxicity Research
|January 1, 1989
Summary
High glucose levels cause rat lenses to develop opacities, indicating cataract formation. New imaging techniques can objectively assess lens function, complementing traditional methods for cataract grading in animal models.
Area of Science:
- Ophthalmology
- Biomedical Engineering
- Animal Models
Background:
- Cataracts are a leading cause of vision loss.
- Accurate grading of cataracts is essential for research and clinical assessment.
- Current grading methods often rely on morphology, potentially missing functional changes.
Purpose of the Study:
- To investigate the functional changes in rat lenses exposed to high glucose.
- To evaluate novel video/computer-based imaging systems for objective lens function assessment.
- To determine if these methods can complement existing cataract grading techniques.
Main Methods:
- Wistar rat lenses were cultured in standard or high-glucose (55 mM) medium.
- Lens opacity development was monitored over 20 hours.
- Two imaging systems were used: Scanning Lens Monitor (laser transmission analysis) and Kevex Image Analysis System (projected white light analysis).
- These systems analyzed changes in focal length and light scattering.
Main Results:
- Glucose-stressed lenses showed opacities within 20 hours, unlike controls.
- The imaging systems successfully detected functional changes, including alterations in focal length and light scattering.
- Combined analysis provided a comprehensive functional assessment.
Conclusions:
- High glucose induces functional changes in rat lenses, preceding visible opacities.
- Video/computer-based imaging systems offer objective, quantitative measures of lens function.
- These techniques can serve as valuable tools for functional cataract grading in animal studies, supplementing morphological assessments.