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Updated: Apr 28, 2026

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.
Published on: June 27, 2025
High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the
Christine M Loescher1, David W Morton1, Slavica Razic2
1School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, VIC, Australia.
Abstract:
Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C. officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition.
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