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Updated: Apr 28, 2026
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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
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A specific fluorescent probe for NO based on a new NO-binding group.
1School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. guow@sxu.edu.cn.
Chemical Communications (Cambridge, England)
|June 3, 2014
Summary
A novel fluorescent probe was developed for detecting nitric oxide (NO). This probe shows high selectivity for NO over other reactive species, enabling precise biological monitoring.
Area of Science:
- Chemical Biology
- Biochemistry
- Analytical Chemistry
Background:
- Nitric oxide (NO) is a crucial signaling molecule.
- Selective detection of NO is challenging due to the presence of other reactive oxygen and nitrogen species (ROS/RNS).
Purpose of the Study:
- To construct a novel fluorescent probe for selective NO detection.
- To evaluate the probe's selectivity against various interfering species.
Main Methods:
- Synthesis of a Bodipy-based fluorescent probe incorporating a 2-amino-3'-dimethylaminobiphenyl NO-binding group.
- Spectroscopic analysis to assess probe performance.
Main Results:
- The developed probe demonstrated high selectivity for nitric oxide (NO).
- The probe showed minimal cross-reactivity with other reactive oxygen and nitrogen species (ROS/RNS), including DHA, AA, and MGO.
Conclusions:
- A selective fluorescent probe for NO detection was successfully synthesized.
- The probe's high selectivity offers a promising tool for studying NO in complex biological systems.
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