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Synthesis of buffers for generating immobilized pH gradients. II: Basic acrylamido buffers
M Chiari1, E Casale, E Santaniello
1Department of Biomedical Science and Technology, University of Milano, Italy.
Applied and Theoretical Electrophoresis : the Official Journal of the International Electrophoresis Society
|January 1, 1989
Abstract:
Six basic acrylamido buffers, for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK greater than 12), QAE-acrylamide, and five weaker bases, namely: 2-morpholinoethyl acrylamide (pK 6.2), 3-morpholinopropyl acrylamide (pK 7.0), N,N-dimethyl aminoethyl acrylamide (pK 8.5), N,N-dimethyl aminopropyl acrylamide (pK 9.3) and N,N-diethyl aminopropyl acrylamide (pK 10.3). Their synthesis and general properties are described.