Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

90.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
90.7K
Spermatogenesis01:22

Spermatogenesis

8.2K
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
8.2K
Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

16.5K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
16.5K
Sperm Transport01:15

Sperm Transport

5.5K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
5.5K
pH Regulation in Cells01:28

pH Regulation in Cells

5.4K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
5.4K
Fertilization01:38

Fertilization

68.7K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
68.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

hSpindly dynamics modulate spindle assembly checkpoint signaling, promoting resistance to mitotic inhibitors.

iScience·2026
Same author

3D+t human sperm flagellum centerline dataset.

Scientific data·2026
Same author

Chloride Intracellular Channel 4 (CLIC4) controls volume regulation in sperm development via Protein Kinase C (PKC).

BMC biology·2026
Same author

Sperm plasma membrane potential as a marker for fertilization success for non-normozoospermic patients undergoing assisted reproduction technologies.

Journal of assisted reproduction and genetics·2026
Same author

Thermal modulation of CatSper activity regulates hyperactivated motility in human sperm†.

Biology of reproduction·2026
Same author

The Relationship Between TikTok Usage and Executive Function Is Mediated by Problematic Social Media Use.

Behavioral sciences (Basel, Switzerland)·2025

Related Experiment Video

Updated: Apr 28, 2026

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

19.7K

Intracellular pH in sperm physiology.

Takuya Nishigaki1, Omar José1, Ana Laura González-Cota1

  • 1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.

Biochemical and Biophysical Research Communications
|June 3, 2014
PubMed
Summary
This summary is machine-generated.

Intracellular pH regulation is crucial for sperm function, involving key transporters like CatSper and Slo3. Understanding these mechanisms is vital for addressing male infertility linked to pH-dependent processes.

Keywords:
Intracellular pHSpermSperm chemotaxisSperm motility

More Related Videos

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
09:30

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Published on: December 30, 2014

16.6K
Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells
09:47

Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells

Published on: February 14, 2021

4.0K

Related Experiment Videos

Last Updated: Apr 28, 2026

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

19.7K
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
09:30

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Published on: December 30, 2014

16.6K
Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells
09:47

Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells

Published on: February 14, 2021

4.0K

Area of Science:

  • Reproductive Biology
  • Cell Physiology
  • Ion Transport Mechanisms

Background:

  • Intracellular pH (pHi) regulation is critical for overall cell function.
  • Several sperm-specific ion transporters and enzymes, essential for fertility, are dependent on or related to pHi regulation.
  • Defects in these pHi-related components can lead to infertility.

Purpose of the Study:

  • To review the key components involved in sperm intracellular pH regulation.
  • To describe the characteristics of these pHi regulatory components.
  • To elucidate their role in fundamental sperm functions.

Main Methods:

  • Literature review of scientific articles on sperm ion transport and pHi.
  • Analysis of the known functions of sperm-specific ion transporters and enzymes.
  • Synthesis of information regarding pHi's role in sperm motility, maturation, and acrosome reaction.

Main Results:

  • Identified key pHi regulatory components in sperm, including CatSper (Ca2+ channel), Slo3 (K+ channel), sperm-specific Na+/H+ exchanger, and soluble adenylyl cyclase.
  • Detailed the characteristics and functions of these identified components.
  • Highlighted the direct involvement of pHi regulation in sperm motility, maturation, and the acrosome reaction.

Conclusions:

  • Intracellular pH regulation is fundamentally important for sperm physiology.
  • Specific ion transporters and enzymes are critical for maintaining cellular pH and enabling key sperm functions.
  • Dysregulation of pHi contributes to male infertility, underscoring the importance of these mechanisms.