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Updated: Apr 28, 2026

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Published on: May 6, 2020
Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA
Dmitriy A Khodakov1, Anastasia S Khodakova, Adrian Linacre
1Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, SA, 5001 Australia. dmitriy.khodakov@flinders.edu.au amanda.ellis@fliders.edu.au.
Abstract:
This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

