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Published on: March 28, 2025
Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts
Ichaya Yiemwattana1, Ruchadaporn Kaomongkolgit2
1Departments of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand. ichayak@yahoo.com.
Abstract:
This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 μg/ml P. gingivalis LPS and alpha-mangostin at 1 µg/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.
Insights
Alpha-mangostin effectively reduces inflammatory markers interleukin-6 and interleukin-8 in human gingival fibroblasts. This suggests alpha-mangostin
Area of Science:
- Periodontology
- Immunology
- Pharmacology
Background:
- Periodontal disease involves inflammation driven by cytokines like IL-6 and IL-8.
- Porphyromonas gingivalis lipopolysaccharide (LPS) is a key trigger for this inflammation.
- Alpha-mangostin is a natural compound with potential anti-inflammatory properties.
Purpose of the Study:
- To investigate the impact of alpha-mangostin on IL-6 and IL-8 expression in human gingival fibroblasts (HGFs).
- To determine the safety and efficacy of alpha-mangostin in modulating inflammatory responses in HGFs challenged with P. gingivalis LPS.
Main Methods:
- Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis LPS.
- Cells were treated with varying concentrations of alpha-mangostin.
- Cytotoxicity was assessed using MTS assay.
- Interleukin (IL)-6 and IL-8 mRNA and protein levels were quantified via Real-time PCR and ELISA.
Main Results:
- Alpha-mangostin at concentrations up to 1 µg/ml did not induce cytotoxicity in HGFs.
- P. gingivalis LPS challenge significantly increased IL-6 and IL-8 expression.
- Alpha-mangostin treatment dose-dependently reduced both IL-6 and IL-8 mRNA and protein levels in LPS-stimulated HGFs.
Conclusions:
- Alpha-mangostin exhibits significant anti-inflammatory effects by suppressing IL-6 and IL-8 production in HGFs.
- These findings support the potential of alpha-mangostin as a therapeutic agent.
- Alpha-mangostin may serve as an effective adjunct in periodontal therapy for managing inflammation.
