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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.3K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.3K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.0K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.0K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

6.8K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
6.8K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

801
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
801
Cell Lines01:16

Cell Lines

8.2K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
8.2K
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.9K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.9K

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Related Experiment Video

Updated: Apr 28, 2026

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

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Live cell NMR.

Darón I Freedberg1, Philipp Selenko

  • 1Laboratory of Bacterial Polysaccharides, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Rockville, Maryland 20852-1448;

Annual Review of Biophysics
|June 5, 2014
PubMed
Summary
This summary is machine-generated.

High-resolution nuclear magnetic resonance (NMR) spectroscopy provides atomic-level insights into live cells without spatial imaging. This powerful technique reveals the native states of cellular biomolecules, advancing structural and functional studies.

Keywords:
excluded volumein-cell EPRin-cell NMRmacromolecular crowdingon-cell NMRsoft interactions

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cells are fundamental units of life, requiring tools to study their internal structures and functions.
  • Optical microscopy is common for cell visualization, but lacks atomic-level detail for biomolecules.
  • Nuclear Magnetic Resonance (NMR) spectroscopy offers a complementary approach for in-depth cellular analysis.

Purpose of the Study:

  • To review recent advancements in in-cell and on-cell NMR spectroscopy.
  • To highlight the capabilities of NMR for studying biomolecules in their native cellular environments.
  • To discuss emerging biological concepts revealed by these NMR techniques.

Main Methods:

  • High-resolution in-cell and on-cell NMR spectroscopy applied to live cells.
  • Analysis of atomic-resolution structural and functional data for biomolecules.
  • Review of current literature and emerging applications of NMR in cell biology.

Main Results:

  • NMR methods provide atomic-resolution insights into proteins, nucleic acids, glycans, and lipids within live cells.
  • These techniques offer a unique view of biomolecular states in their native cellular context.
  • NMR bypasses the spatial limitations of imaging techniques for molecular-level understanding.

Conclusions:

  • In-cell and on-cell NMR spectroscopy are powerful tools for elucidating cellular mechanisms at the molecular level.
  • These methods complement traditional imaging, offering unparalleled detail on biomolecular structure and function.
  • Emerging concepts in cell biology are being uncovered through these advanced NMR applications.