Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sustained Reduction in Cardiopulmonary Fitness in Long COVID: A Report from the RECOVER-adult Cohort Study.

JACC. Advances·2026
Same author

Whole-protein screening and multi-modal profiling of antigen-specific CD4<sup>+</sup> T cells at single-cell resolution.

Nature communications·2026
Same author

Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.

Nature communications·2026
Same author

Long COVID trajectories in the prospectively followed RECOVER-Adult US cohort.

Nature communications·2025
Same author

School-Based Interventions for Reducing Disciplinary School Exclusion. An Updated Systematic Review.

Campbell systematic reviews·2025
Same author

Cytokines Associated With Moderate and Severe Adverse Events During a Sporozoite Malaria Vaccine Trial With Controlled Human Malaria Infection.

The Journal of infectious diseases·2025

Related Experiment Video

Updated: Apr 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.5K

Microfluidics-based single-cell functional proteomics for fundamental and applied biomedical applications.

Jing Yu1, Jing Zhou, Alex Sutherland

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|June 5, 2014
PubMed
Summary

Microfluidics platforms enable advanced single-cell functional proteomics by simultaneously assaying over 20 proteins. This technology allows correlation of cell behaviors with protein expression for deeper biological insights.

Keywords:
immunoassaysmicrofluidicsprotein signalingsingle-cell proteomics

More Related Videos

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.1K
Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.2K

Related Experiment Videos

Last Updated: Apr 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.5K
Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.1K
Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.2K

Area of Science:

  • Biotechnology
  • Proteomics
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity and function.
  • Traditional proteomics methods often require large cell populations, obscuring individual cell behavior.
  • Emerging microfluidics technologies offer novel solutions for high-throughput single-cell protein analysis.

Purpose of the Study:

  • To review the emerging microfluidics-based toolkit for single-cell functional proteomics.
  • To discuss the advantages, design concepts, and applications of microfluidic platforms in this field.
  • To explore the future directions and potential of microfluidics in biomedical research.

Main Methods:

  • Review of existing literature on microfluidics for single-cell functional proteomics.
  • Discussion of microfluidic platform design principles and their capabilities.
  • Analysis of reported applications in cell behavior, signaling networks, and cell-cell interactions.

Main Results:

  • Microfluidics enables simultaneous assaying of 20+ functional proteins from single cells.
  • Platforms allow correlation of cell behaviors (e.g., motility) with protein expression.
  • Capabilities include analyzing cell-cell interactions, identifying rare cells, and bridging biology with physical laws.

Conclusions:

  • Microfluidics-based single-cell functional proteomics offers significant advantages over traditional methods.
  • These platforms are valuable tools for dissecting complex biological systems and have broad biomedical applications.
  • The field holds great promise for advancing our understanding of cellular function and disease.