Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

90
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

2.0K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.0K
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

215
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
215
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.0K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.0K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

1.4K
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
1.4K
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

76
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
76

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation and characterization of bacteriocin-producing E. coli isolates from a poultry slaughterhouse, and cell-free production and evaluation of native and engineered bacteriocins.

Poultry science·2025
Same author

Saprophyticin S: A Novel Saposin-like, Leaderless Bacteriocin Produced by Staphylococcus saprophyticus P1CEA4 of Porcine Origin.

Probiotics and antimicrobial proteins·2025
Same author

Exploring the Functional Potential of the Broiler Gut Microbiome Using Shotgun Metagenomics.

Genes·2025
Same author

Multiplexed bacteriocin synthesis to combat and prevent antimicrobial resistance.

Communications biology·2025
Same author

Screening and Genomic Profiling of Antimicrobial Bacteria Sourced from Poultry Slaughterhouse Effluents: Bacteriocin Production and Safety Evaluation.

Genes·2025
Same author

Insights into ecology, pathogenesis, and biofilm formation of <i>Enterococcus faecalis</i> from functional genomics.

Microbiology and molecular biology reviews : MMBR·2024
Same journal

Vision and Development of a Design, Implementation, and Verification Automation (DIVA) Software Platform for DNA Construction.

ACS synthetic biology·2026
Same journal

Engineering a Cytochrome P450 <i>O</i>-Demethylase for the Bioconversion of Hardwood Lignin.

ACS synthetic biology·2026
Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026
Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026
Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026
Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026
See all related articles

Related Experiment Video

Updated: Apr 28, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.3K

Modified lactic acid bacteria detect and inhibit multiresistant enterococci.

Juan Borrero1, Yuqing Chen1, Gary M Dunny1

  • 1†Department of Chemical Engineering and Materials Science, ‡Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, United States.

ACS Synthetic Biology
|June 5, 2014
PubMed
Summary
This summary is machine-generated.

Engineered Lactococcus lactis detects Enterococcus faecalis using a specific signal. Upon detection, it produces antimicrobial peptides that inhibit enterococcal growth, offering a novel approach for infection control.

Keywords:
Enterococcus faecalisLactococcus lactisdrug delivery vectorlactic acid bacteriarecombinant plasmids

More Related Videos

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
07:54

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

13.6K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

3.0K

Related Experiment Videos

Last Updated: Apr 28, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.3K
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
07:54

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

13.6K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

3.0K

Area of Science:

  • Microbiology
  • Synthetic Biology
  • Antimicrobial Research

Background:

  • Enterococcus species are significant human pathogens, often exhibiting multidrug resistance.
  • Current antibiotic treatments face challenges due to rising resistance, necessitating alternative strategies.
  • Targeting infections at the site requires precise detection and localized inhibition.

Purpose of the Study:

  • To engineer Lactococcus lactis as a biosensor for Enterococcus faecalis detection.
  • To develop a system for localized antimicrobial peptide secretion upon pathogen detection.
  • To explore alternative antimicrobial technologies against enterococci, including multidrug-resistant strains.

Main Methods:

  • Engineered Lactococcus lactis with expression vectors derived from the pCF10 plasmid.
  • Constructed recombinant strains to express bacteriocins: enterocin A, hiracin JM79, and enterocin P.
  • Utilized the enterococcal sex pheromone cCF10 as the specific detection signal.

Main Results:

  • Engineered L. lactis successfully detected E. faecalis upon induction by cCF10.
  • Secreted bacteriocins inhibited enterococcal growth and reduced viability in vitro.
  • The system demonstrated efficacy against multidrug-resistant Enterococcus faecium strains.

Conclusions:

  • Engineered L. lactis serves as a sensitive and specific biosensor for E. faecalis.
  • The developed bacteriocin-producing strains offer a targeted antimicrobial approach.
  • This platform provides components for a toolbox for alternative antibiotic development against enterococci.