Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic and serological analyses of equine influenza viruses isolated in Kumamoto and Hokkaido, Japan in 2025.

Veterinary microbiology·2025
Same author

Multiple introductions of equine influenza virus into the United Kingdom resulted in widespread outbreaks and lineage replacement.

PLoS pathogens·2025
Same author

A comparative evaluation of seven commercial human influenza virus antigen detection kits for the diagnosis of equine influenza.

Equine veterinary journal·2025
Same author

Protective efficacy of a bivalent equine influenza H3N8 virus-like particle vaccine in horses.

Vaccine·2025
Same author

Should the equine community be concerned about the emergence of the H5N1 subtype of highly pathogenic avian influenza in US cattle?

Equine veterinary journal·2024
Same author

Effect of dexamethasone on antibody response of horses to vaccination with a combined equine influenza virus and equine herpesvirus-1 vaccine.

Journal of veterinary internal medicine·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 28, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
09:57

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

31.7K

Equine influenza serological methods.

Thomas M Chambers1, Stephanie E Reedy

  • 1Department of Veterinary Science, OIE Reference Laboratory for Equine Influenza, Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546-0099, USA, tmcham1@uky.edu.

Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2014
PubMed
Summary
This summary is machine-generated.

Serologic tests like hemagglutination-inhibition (HI) and single radial hemolysis (SRH) are crucial for equine influenza virus (EIV) antibody detection. These methods aid in diagnosis, strain comparison, and vaccine response assessment.

More Related Videos

High-throughput Detection Method for Influenza Virus
10:05

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

24.2K
An Optimized Hemagglutination Inhibition HI Assay to Quantify Influenza-specific Antibody Titers
06:34

An Optimized Hemagglutination Inhibition HI Assay to Quantify Influenza-specific Antibody Titers

Published on: December 1, 2017

36.1K

Related Experiment Videos

Last Updated: Apr 28, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
09:57

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

31.7K
High-throughput Detection Method for Influenza Virus
10:05

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

24.2K
An Optimized Hemagglutination Inhibition HI Assay to Quantify Influenza-specific Antibody Titers
06:34

An Optimized Hemagglutination Inhibition HI Assay to Quantify Influenza-specific Antibody Titers

Published on: December 1, 2017

36.1K

Area of Science:

  • Veterinary Virology
  • Immunology
  • Diagnostic Assays

Background:

  • Serologic tests are essential for equine influenza virus (EIV) antibody detection.
  • Applications include retrospective diagnosis, virus subtyping, antigenic comparison, and vaccine response assessment.

Purpose of the Study:

  • To describe widely used serologic tests for equine influenza virus antibodies.
  • To highlight methodologies and potential challenges in antibody detection.

Main Methods:

  • Detailed description of hemagglutination-inhibition (HI), single radial hemolysis (SRH), and serum micro-neutralization tests.
  • Discussion of methods for removing hemagglutination inhibitors in equine serum (e.g., receptor-destroying enzyme, periodate, trypsin-periodate).
  • Mention of antigen pretreatment protocols (Tween-80, ether) for EIV H3N8 strains.

Main Results:

  • HI, SRH, and serum micro-neutralization are the most common assays.
  • Equine serum inhibitors can complicate HI assay interpretation.
  • SRH assay shows stronger correlation between antibody titers and disease protection.

Conclusions:

  • Established serologic tests provide valuable data for equine influenza virus surveillance and research.
  • SRH may offer a more reliable indicator of protective immunity compared to HI.
  • Specialized assays exist for specific applications like neuraminidase-inhibition and ELISA.