Related Experiment Video
Updated: Aug 4, 2026
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Determination of sulphated glycosaminoglycans by automated potentiometric titration with simple coated-wire
1Department of Analytical Chemistry and Biochemistry, Research Institute of Pharmacy and Biochemistry, Vinohrady, Czechoslovakia.
Abstract:
A method for the determination of sulphated glycosaminoglycans is based on their precipitation with (1-ethoxycarbonyl)pentadecyltrimethylammonium bromide (Septonex), the excess of which is back-titrated with sodium tetraphenylborate. The titration is monitored by a simple coated-wire ion-selective electrode with a plasticized poly(vinyl chloride) membrane on aluminium wire. Under certain conditions the results are almost independent of the relative molecular mass of glycosaminoglycans. The method has been applied to the determination of the active ingredient in the pharmaceutical preparation, heparon injection.
