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Exopolysaccharide (EPS) synthesis by Oenococcus oeni: from genes to phenotypes
Maria Dimopoulou1, Marlène Vuillemin2, Hugo Campbell-Sills1
1Université de Bordeaux, Institut polytechnique de Bordeaux, ISVV, EA 4577, Unité de recherche Oenologie, INRA USC 1366, Villenave d'Ornon, France.
Plos One
|June 6, 2014
Summary
This study analyzes Oenococcus oeni genomes, identifying genes for exopolysaccharide (EPS) production. Different pathways contribute to EPS diversity, crucial for wine bacteria adaptation and malolactic starter performance.
Area of Science:
- Microbiology
- Genomics
- Biochemistry
Background:
- Oenococcus oeni is key for malolactic fermentation in wine.
- Exopolysaccharides (EPS) produced by O. oeni influence wine characteristics and starter culture performance.
- Understanding EPS biosynthesis is crucial for wine quality and microbial management.
Purpose of the Study:
- To identify and characterize genes involved in exopolysaccharide (EPS) biosynthesis in Oenococcus oeni.
- To correlate EPS genotypes with observed phenotypes and structures.
- To investigate the distribution and potential roles of EPS biosynthesis genes in O. oeni populations.
Main Methods:
- Genomic sequencing and analysis of 50 O. oeni strains.
- Identification of gene clusters (eps1, eps2) and isolated genes (dsrO, dsrV, levO, gtf, it3, it4) related to EPS synthesis.
- Phenotypic analysis of EPS production and structure determination under various growth conditions.
Main Results:
- Inventory of 50 O. oeni genomes revealed diverse EPS biosynthesis gene clusters and isolated genes.
- Multiple EPS biosynthetic pathways were identified: Wzy-dependent (heteropolysaccharides), glucan synthase (β-glucan), and glycoside-hydrolase-mediated (α-glucan, β-fructan).
- EPS gene distribution varied among strains, with all genomes possessing genes for EPS metabolism, suggesting their importance.
Conclusions:
- Oenococcus oeni possesses diverse and complementary EPS biosynthetic pathways.
- EPS production is a significant trait for O. oeni adaptation to the wine environment.
- EPS likely contributes to the technological performance of malolactic starter cultures.
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