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Updated: Apr 28, 2026

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Published on: February 8, 2021
Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy:
Wen-Jing Bao1, Zhen-Dong Yan, Min Wang
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China. xhxia@nju.edu.cn.
Abstract:
Distance-dependent signal intensity in immunoassay by attenuated total reflection-surface enhanced infrared absorption spectroscopy is demonstrated by controlling the distance of target proteins away from the enhancement substrate. Based on this optical near-field effect, sensitive detection of protein molecules with a detection limit of 0.6 nM and investigation of the kinetics and thermodynamics of protein-aptamer/antibody interactions can be achieved.
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