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Related Concept Videos

Muscle Stimulation Frequency01:22

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Wave summation
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Exercise induces a range of adaptations in muscle tissue, depending on the type and duration of activity. Such physical training can be broadly categorized into two types: endurance exercises and resistance exercises.
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Related Experiment Video

Updated: Apr 28, 2026

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
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Muscle echo intensity: reliability and conditioning factors.

Cristina Caresio1, Filippo Molinari2, Giorgio Emanuel1

  • 1Division of Endocrinology, Diabetology and Metabolism, Department of Medical Sciences, University of Turin, Turin, Italy.

Clinical Physiology and Functional Imaging
|June 7, 2014
PubMed
Summary

Muscle echo intensity reliability depends on region of interest (ROI) size. Factors like muscle type, gender, and subcutaneous fat influence echo intensity measurements in ultrasound assessments.

Keywords:
fibrous and adipose tissue content of musclesgreyscale analysismuscle thicknessregion of interestsubcutaneous layer thickness

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Area of Science:

  • Musculoskeletal Ultrasound
  • Biomedical Imaging
  • Human Physiology

Background:

  • Muscle echo intensity is a quantitative ultrasound parameter.
  • Reliability of echo intensity measurements is crucial for clinical applications.
  • Understanding factors affecting echo intensity is important for accurate assessment.

Purpose of the Study:

  • To evaluate the reliability of muscle echo intensity measurements.
  • To examine the influence of region of interest (ROI) parameters (size, shape, location) on echo intensity.
  • To investigate correlations between muscle echo intensity and anthropometric factors.

Main Methods:

  • Ultrasound scans of five muscles (biceps brachii, rectus femoris, vastus lateralis, tibialis anterior, medial gastrocnemius) were performed on 20 healthy subjects.
  • Muscle echo intensity was quantified using various region of interest (ROI) sizes, shapes, and locations.
  • Intraclass correlation coefficients (ICC) were used to assess reliability and consistency.

Main Results:

  • Reliability varied from moderate (ICC: 0.54) to very high (ICC: 0.91) depending on ROI size and scan consistency.
  • Specific muscles (tibialis anterior, rectus femoris) showed significant differences in echo intensity based on ROI characteristics.
  • Higher echo intensity was observed in biceps brachii and tibialis anterior, in females compared to males, and correlated positively with subcutaneous fat thickness in some muscles.

Conclusions:

  • Muscle echo intensity reliability is significantly influenced by the size of the ROI.
  • Variations in muscle echo intensity across muscles and genders are likely attributed to differences in fibrous and adipose tissue composition.
  • The correlation between muscle echo intensity and subcutaneous fat thickness may relate to overall adiposity or intramuscular fat content.