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Updated: Apr 28, 2026
Conjunctival Commensal Isolation and Identification in Mice
Published on: May 1, 2021
Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis
Huimin Liu1,2,3,4,5, Jing Yan1,2,3,4,5, Yutian Wang1,2,3,4,5
1National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.
Abstract:
Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive. The sequence of the 16S ribosomal DNA (rDNA) gene of the isolates matched the 16S rDNA sequence of M. bovoculi. Amplification of the 16S-23S intergenic spacer region followed by AfaI digestion produced a 600-base pair product, a result characteristic of M. bovoculi isolates. The phylogenetic analysis based on the 16S rDNA sequence confirmed the strain isolated in the current study had genetic homology with M. bovoculi.
