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The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
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Expression pattern of long non-coding RNAs in renal cell carcinoma revealed by microarray.
Chao Qin1, Zhijian Han1, Jian Qian1
1Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Plos One
|June 7, 2014
Summary
This study investigated long non-coding RNAs (lncRNAs) in renal cell carcinoma (RCC), finding many aberrant lncRNAs and mRNAs. AK096725 expression was notably associated with RCC, suggesting potential new biomarkers.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Large-scale transcriptome analyses reveal aberrant long non-coding RNAs (lncRNAs) in various diseases, including cancers.
- The specific involvement of lncRNAs in renal cell carcinoma (RCC) remains unclear.
- Investigating lncRNA expression patterns in RCC is crucial for understanding disease mechanisms.
Purpose of the Study:
- To investigate the expression patterns of lncRNAs in renal cell carcinoma (RCC) tumor samples compared to adjacent non-tumor tissues.
- To identify specific deregulated lncRNAs and messenger RNAs (mRNAs) in RCC.
- To explore the potential of identified lncRNAs as biomarkers for RCC.
Main Methods:
- Utilized microarray analysis with 33,045 lncRNA and 30,215 mRNA probes.
- Analyzed five paired RCC tumor and adjacent non-tumor tissue samples.
- Confirmed expression levels of specific lncRNAs (AK096725 and ENST00000453068) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 70 paired samples.
Main Results:
- Microarray analysis revealed 27,279 lncRNAs in RCC samples, with 480 upregulated and 417 downregulated.
- Detected 19,995 mRNAs, of which 458 were upregulated and 413 downregulated.
- Expression changes of AK096725 and ENST00000453068 in 70 paired samples validated microarray findings.
Conclusions:
- Uncovered distinct lncRNA expression patterns in RCC tumor tissues compared to non-tumor tissues.
- Identified numerous aberrant lncRNAs and mRNAs associated with RCC.
- Highlighted the significant association between AK096725 expression and RCC, suggesting its potential as a novel biomarker.
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